The 12-volume set LNCS 15001-15012 constitutes the proceedings of the 27th International Conferenc on Medical Image Computing and Computer Assisted Intervention, MICCAI 2024, which took place in Marrakesh, Morocco, during October 6th-10th, 2024. MICCAI accepted 857 full papers from 2781 submissions. They focus on neuroimaging; image registration; computational pathology; computer aided diagnosis, treatment response, and outcome prediction; image guided intervention; visualization; surgical planning, and surgical data science; image reconstruction; image segmentation; machine learning; etc.