Part I: Basic methods: Maintenance of stem cell cultures 1. Human Embryonic Stem Cell Culture 2. Feeder layers and feeder-free culture 3. Mouse Embryonic Fibroblast Feeder Cells 4. Cryopreservation of hESC Part II: Basic methods: Characterization of stem cells 5. Classical Cytogenetics: Karyotyping 6. Spectral Karyotyping and Fluorescent in situ Hybridization 7. Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns 8.

FACS/Cell sorting 9. Fluorescence Immunocytochemical Analysis of Stem Cells 10. Characterization of Stem Cells Using RT-PCR 11. Gene Expression Profiling of Stem Cells by Microarray 12. Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice 13. Generation of HESC-Derived Teratomas Part III: Differentiation of stem cells 14. "Embryoid Body" Formation and Neuroepithelia Differentiation 15. Motor Neuron and Dopamine Neuron Differentiation 16.

Oligodendrocyte Differentiation from hESC 17. Cardiac 18. Hematopoietic Part IV: Genetic manipulation of stem cells 19. Genetic Manipulation of hESC: Lentivirus Vectors 20. Methods developed for mouse ES cells Part V: Advanced methods 21. Derivation of human ES cells 22. Neural Stem Cell Culture 23. Transplantation of stem cells 24.

In vitro fertilization 25. Functional characterization-neural cells Part VI: Practical issues in establishing a human ES cell laboratory 26. Setting Up a Facility for hESC Research Part VII: Essays on stem cell biology 27. Intellectual Property: Owning the Stem Cell 28. Ethical Concerns for Stem Cell Research 29. Guidelines for hESC Research Oversight (ESCRO) Committees.