More than 350 problems in the book now demonstrate how the fundamental principles are applied in areas such as biofuels, bioplastics, bioremediation, tissue engineering, site-directed mutagenesis, recombinant protein production, and drug development, as well as for traditional microbial fermentation Provides in-depth treatment of fluid flow, turbulence, mixing, and impeller design, reflecting recent advances in our understanding of mixing processes and their importance in determining the performance of cell cultures Focuses on underlying scientific and engineering principles rather than on specific biotechnology applications, providing a sound basis for teaching bioprocess engineering Topics such as fluid flow and mixing, heat and mass transfer, reaction engineering, and downstream processing are presented in detail as they apply to bioprocessing Includes new or expanded coverage of such topics as enzyme kinetics, downstream processing, disposable reactors, genetic engineering, and the technology of fermentation.