1. Enzymes -Their place in Biology.- 2. Enzymes - Historical aspects.- 3. Hallmarks of an enzyme catalyst.- 4. Origins of enzyme catalytic power.

- 5. Which enzyme uses what tricks?.- 6. Structure and catalysis: Conformational flexibility and protein motion.- 7. On enzyme nomenclature and classification.- 8. Chemical Kinetics: Fundamentals.

- 9. Concepts of equilibrium and steady state.- 10. ES Complex and Pre-steady-state Kinetics.- 11. Principles of Enzyme Assays.- 12. Good Kinetic Practices.

- 13. Quantification of Catalysis and Measures of Enzyme Purity.- 14. Henri-Michaelis-Menten Equation.- 15. More Complex Rate Expressions.- 16. Enzyme Kinetic Data: Collection and Analysis.

- 17. Approaches to Kinetic Mechanism: An Overview.- 18. Analysis of Initial Velocity Patterns.- 19. Enzyme Inhibition Analyses.- 20. Irreversible Inhibitions.

- 21. Reversible Inhibitions.- 22. Alternate Substrate (Product) Interactions.- 23. pH Studies with Enzymes.- 24. Isotopes in Enzymology.

- 25. Isotope Effects in Enzymology.- 26. From Kinetic Data to Mechanism and Back.- 27. Chemical Reactivity and Molecular Interactions.- 28. Acid-Base Chemistry and Catalysis.

- 29. Nucleophilic Catalysis and Covalent Reaction Intermediates.- 30. Phosphoryl Group Chemistry and Importance of ATP.- 31. Enzymatic Oxidation-Reduction Reactions.- 32. Carboxylations and Decarboxylations.

- 33. Electrophilic Catalysis and Amino Acid Transformations.- 34. Free Radicals and radical enzymology.- 35. Integrating Kinetic and Chemical Mechanisms: A Synthesis.- 36. Enzyme Inhibitor Design: Drug Discovery.

- 37. Exploiting enzymes: Technology and applications.- 38. Regulation of Enzyme Activity.- 39. In Vitro Versus In Vivo: Concepts and Consequences.- 40. Future of Enzymology: An Appraisal.