Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. Huge application potential of therapeutic and industrial proteins has led to increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins. Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into 9 chapters in a logical fashion beginning with an introduction to recombinant proteins and moving onto expression in different host expression systems followed by extraction, purification, and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place; and finally ends with different characterization techniques.
Fundamentals of Recombinant Protein Production, Purification and Characterization