Chapter 1. Enzymatic processes as a basis for the creation of advanced chemical catalysts. 1.1 A brief overview of the role of enzymes in metabolism, biotechnology and medicine1.2 Enzymatic systems of particular interest for chemical mimicry. Key enzymes (in brackets)1.2.x Photosystem 2 (rection center), Photosystem 1 (oxygen evolving complex), nitrogen fixation (nitrogenase), methane oxidation (methane mono oxygenase), carbon dioxide reduction (formate dehydrogenase), enzymatic hydrolysis of cellulose to glucose (cellulase ), nitric monoxide synthesis ( nitric oxide synthase ), hydrolysis of proteins (protases), covid biochemistry (secreted phospholipase A2 group IIA (sPLA2-IIA).

Chapter 2 Methods of enzyme kinetics 2.1 Analysis of substrates, products and intermediates. Physical grounds and sensitivity 2.2 Ultraviolet-Visible Spectroscopy, Fluorescence, Mass-spectrometry, Nuclear Magnetic Resonance (NMR) , Magnetic resonance spectroscopy imaging (MRI) Electron Spin resonance (ESR), Calorimetry, Thermometry, Amperometry, Potentiometry 2.3 Methods for measuring rate and equilibrium constants 2.4 Steady state, rapid mixing continuous flow, stopped flow, quench Flow, zone trapping/bypass zone trapping flow, air-segmented flow, concentration-jump the temperature-jump, pressure jump, jumplaser-induced potential jump pressure, photoswitching Chapter 3 Elementary mechanism of key chemical steps of enzyme reactions 3.1 Long distance electron transfer 3.1.

1 Theory 3.1.2 Examples 3.2 Cascade electron transfer in conversion light energy processes 3.3 Concert reactions 3.3.1Theory 3.3.

1.1 Energy factors 3.3.1.2 Synchronization factor. Principle of nuclear optimum motion 3.3.1.

3 Examples 3.4 Multi-electron redox processes 3.4.1 Theoretical considerations 3.4.2 Examples Chapter 4 Factors affected on enzyme catalysis. Theoretical grounds and examples 4.1 Precise orientation 4.

2 Protein molecular dynamics, and conformational transition. Principle of dynamical adaption.