Part I Basic Fluorescence Imaging 1 Fluorescence Microscopy: A Field Guide for Biologists. Lucy Swift and Pina Colarusso 2 Three-Dimensional Simultaneous Imaging of Nucleic Acids and Proteins During Influenza Virus Infection in Single Cells Using Confocal Microscopy. Richard Manivanh, Seema S. Lakdawala, and Jennifer E. Jones 3 Optimizing Long-Term Live Cell Imaging. Alex Lac, Austin Le Lam, and Bryan Heit Part II Quantitative Approaches 4 Monitoring Transmembrane and Peripheral Membrane Protein Interactions by Förster Resonance Energy Transfer Using Fluorescence Lifetime Imaging Microscopy. Janhavi Nagwekar, Caterina Di Ciano-Oliveira, and Gregory D. Fairn 5 Bimolecular Fluorescence Complementation to Visualize Protein:Protein Interactions in Cells.
Cassandra R. Edgar and Jimmy D. Dikeakos 6 Monitoring Cellular Responses to Infection with Fluorescent Biosensors. Amena Aktar, Kasia M Wodz, and Bryan Heit 7 Quantifying Endothelial Transcytosis with Total Internal Reflection Fluorescence Microscopy (TIRF). Erika Jang, Siavash Ghaffari, and Warren L. Lee 8 Measurement of Minute Cellular Forces by Traction Force Microscopy. Valentin Jaumouillé Part III Tissue and Live Animal Imaging 9 Quantitative Immunofluorescent Imaging of Immune Cells in Mucosal Tissues. Lane B.
Buchanan, Zhongtian Shao, Yuan Chung Jiang, Abbie Lai, Thomas J. Hope, Ann M. Carias, and Jessica L. Prodger 10 Intravital Microscopy Techniques to Image Wound Healing in Mouse Skin. Madison Turk, Jeff Biernaskie, Douglas J. Mahoney, and Craig N. Jenne 11 Quantifiable Intravital Light Sheet Microscopy. Holly C.
Gibbs, Sreeja Sarasamma, Oscar R. Benavides, David G. Green, Nathan A. Hart, Alvin T. Yeh, Kristen C. Maitland, and Arne C. Lekven 12 Hydrophobic and Hydrogel-based Methods for Passive Tissue Clearing. Frank L.
Jalufka, Sun Won Min, Madison E. Platt, Anna L. Pritchard, Theodore E. Margo, Alexandra O. Vernino, Megan A. Kirchhoff, Ryan T. Massopust, and Dylan A. McCreedy 13 Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos.
Ory Perelsman, Shoh Asano, and Limor Freifeld Part III Super-Resolution Approaches 14 Super Resolution Radial Fluctuations (SRRF) Microscopy. Jayme Salsman and Graham Dellaire 15 A Practical Guide for STED Imaging. Walaa Alshafie and Thomas Stroh. 16 Single-Molecule Localization Microscopy of Subcellular Protein Distribution in Neurons. Jelmer Willems, Manon Westra, and Harold D. MacGillavry 17 Measuring the Lateral Diffusion of Plasma Membrane Receptors using Raster Image Correlation Spectroscopy. Sara Makaremi and Jose Moran-Mirabal 18 Nanometer-Scale Molecular Mapping by Super-Resolution Fluorescence Microscopy. Vito Mennella and Zhen Liu Part IV Micrograph Analysis 19 Visualizing and Quantifying Data from Timelapse Imaging Experiments.
Eike K. Mahlandt and Joachim Goedhart 20 Automated Segmentation and Microscopy Image Analysis with Machine Learning Anthony Bilodeau, Catherine Bouchard, and Flavie Lavoie-Cardinal.