Part I: Overview on Mouse and Human Dendritic Cells 1. Origin, Phenotype, and Function of Mouse Dendritic Cell Subsets Dorothée Duluc and Vanja Sisirak 2. Phenotypes and Functions of Human Dendritic Cell Subsets Particularly in the Tumor Microenvironment Candice Sakref, Nathalie Bendriss-Vermare, and Jenny Valladeau-Guilemond Part II: In Vivo Study of Dendritic Cells 3. In Vivo Tracking of Dendritic Cell Migration Michio Tomura 4. In Vivo Analysis of Dendritic Cell Clonality Mar Cabeza-Cabrerizo Part III: In Vitro Differentiation of Dendritic Cells 5. In Vitro Generation of Murine Bone Marrow-Derived Dendritic Cells Yohan Gerber-Ferder, Pierre Bourdely, Mathias Vetillard, Pierre Guermonprez, and Julie Helft 6. In Vitro Generation of Murine Dendritic Cells from Hoxb8-Immortalized Hematopoietic Progenitors Hans Häcker 7. In Vitro Generation of Murine CD8α+ DEC205+ XCR1+ Cross-Presenting Dendritic Cells from Bone Marrow-Derived Hematopoietic Progenitors Margaret E.

Kirkling and Boris Reizis 8. In Vitro Generation of Human Dendritic Cell Subsets from CD34+ Cord Blood Progenitors Pierre Bourdely, Roberto Savoldelli, Mathias Vetillard, Giorgio Anselmi, Julie Helft, and Pierre Guermonprez 9. In Vitro Generation of Human Cross-Presenting Type 1 Conventional Dendritic Cells (cDC1s) and Plasmacytoid Dendritic Cells (pDCs) Xinlong Luo, Sreekumar Balan, Catharina Arnold-Schrauf, and Marc Dalod 10. Culture System Allowing the Simultaneous Differentiation of Human Monocytes into Dendritic Cells and Macrophages Using M-CSF, IL-4, and TNF- a Javiera Villar, Alice Coillard, Corné van Roessel, and Elodie Segura 11. Clonal Analysis of Human Dendritic Cell Progenitors Using a Stromal Cell Culture Kang Liu, Jaeyop Lee, and Thomas Luh Part IV: Enrichment of Dendritic Cells 12. Enrichment of Large Numbers of Splenic Mouse Dendritic Cells after Injection of Flt3L-Producing Tumor Cells Pauline Santa, Anaïs Roubertie, Séverine Loizon, Anne Garreau, Amandine Ferriere, Dorothée Duluc, and Vanja Sisirak 13. Isolation and Identification of Dendritic Cell Subsets from Human and Mouse Tumors Yamila Rocca, Aurélien Voissière, Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare Part V: Functional Characterization of Dendritic Cells 14. Optimized Non-Viral Gene Disruption in Primary Murine and Human Myeloid Cells Emily C.

Freund, Simone M. Haag, Benjamin Haley, and Aditya Murthy 15. Monitoring the Interaction between Dendritic Cells and T Cells In Vivo with LIPSTIC Giulia Pasqual, Aleksey Chudnovskiy, and Gabriel D. Victora 16. Characterization of Dendritic Cell Metabolism by Flow Cytometry Eline C. Brombacher, Thiago A. Patente, Marjolein Quik, and Bart Everts 17. In Vivo and In Vitro Assay to Address Dendritic Cells Antigen Cross-Presenting Capacity Pengju Ou, Lifen Wen, Hai Ni, and Cliff.

Y. Yang 18. Assays to Detect Cross-Dressing by Dendritic Cells In Vivo and In Vitro Alok Das Mohapatra and Pramod K. Srivastava 19. Assessing the Ability of Human Dendritic Cells to Stimulate Naive CD4+ and CD8+ T Cells Alice Coillard, Tsing-Lee Tang-Huau, and Elodie Segura 20. In Vitro and In Vivo Assays to Evaluate Dendritic Cell Phagocytic Capacity Lucie Maisonneuve and Bénédicte Manoury 21. Tracking Plasmacytoid Dendritic Cell Response to Physical Contact with Infected Cells Margarida Sá Ribeiro, Garima Joshi, Elodie Décembre, Célia Nuovo, Adrien Bosseboeuf, Alicia Bellomo, Manon Venet, Sonia Assil, and Marlène Dreux Part VI: Omic Approaches to Study Dendritic Cells 22. Harnessing Single Cell RNA Sequencing to Identify Dendritic Cell Types, Characterize Their Biological States, and Infer Their Activation Trajectory Ammar Sabir Cheema, Kaibo Duan, Marc Dalod, and Thien-Phong Vu Manh 23.

Characterization of Developmental Trajectories of Dendritic Cell Hematopoiesis through Single Cell RNA Sequencing Methods Regine J. Dress and Florent Ginhoux.