This resource examines biotransformation and enzymes, focusing on their crucial roles in biology and biotechnology. It is intended for students, researchers, and professionals who are interested in understanding the biochemical processes by which organisms metabolize various compounds and how enzymes facilitate these processes. The primary aim is to provide a clear connection between theoretical concepts and their practical applications, showcasing biotransformation as an essential tool for developing sustainable solutions in pharmaceuticals, environmental management, and industrial practices. It covers various biotransformation reactions, highlighting their importance in producing valuable products, detoxifying substances, and minimising waste.