Introduction Systems Biology, Biological Knowledge, and Kinetic Modelling Dependence of enzyme reaction rate on the substrate concentration What are the model limitations? Or, in other words, what can be modeled? Cellular Networks Reconstruction and Static Modelling Pathway reconstruction The high-quality network reconstruction: description of the process Visual notations: three categories Edinburgh Pathway Editor (EPE) Introduction Feature summary of EPE A flexible visual representation Conclusion Construction and Verification of Kinetic Models Introduction Basic principles of kinetic model construction Basic principles of kinetic model verification Study of dynamic and regulatory properties of the kinetic model Introduction to DBSolve Creation and analysis of the models using DBSolve: functional description Enzyme Kinetics Modelling Introduction Basic principles of modeling individual enzymes and transporters "Hyperbolic" enzymes Allosteric enzymes Transporters Kinetic Models of Biochemical Pathways Modelling of the mitochondrial Krebs cycle Modelling of the Escherichia colibranched-chain amino acid biosynthesis Modelling of Mitochondrial Energy Metabolism Oxidative phosphorylation and superoxide production in mitochondria Development of kinetic models Description of individual processes of the model Model predictions Application of the Kinetic Modelling Approach to Problems in Biotechnology and Biomedicine Study of the mechanisms of salicylate-hepatotoxic effect Multiple target identification analysis for antituberculosis drug discovery Application of the kinetic model of E. colibranched-chain amino acid biosynthesis to optimize production of isoleucine and valine Conclusion and Discussion References Index sis of the models using DBSolve: functional description Enzyme Kinetics Modelling Introduction Basic principles of modeling individual enzymes and transporters "Hyperbolic" enzymes Allosteric enzymes Transporters Kinetic Models of Biochemical Pathways Modelling of the mitochondrial Krebs cycle Modelling of the Escherichia colibranched-chain amino acid biosynthesis Modelling of Mitochondrial Energy Metabolism Oxidative phosphorylation and superoxide production in mitochondria Development of kinetic models Description of individual processes of the model Model predictions Application of the Kinetic Modelling Approach to Problems in Biotechnology and Biomedicine Study of the mechanisms of salicylate-hepatotoxic effect Multiple target identification analysis for antituberculosis drug discovery Application of the kinetic model of E. colibranched-chain amino acid biosynthesis to optimize production of isoleucine and valine Conclusion and Discussion References Index of the mechanisms of salicylate-hepatotoxic effect Multiple target identification analysis for antituberculosis drug discovery Application of the kinetic model of E. colibranched-chain amino acid biosynthesis to optimize production of isoleucine and valine Conclusion and Discussion References Index.