1 Genes and chromatin 1.1 Central dogma of molecular biology 1.2 Impact of chromatin structure 1.3 Epigenetics enables gene expression 1.4 Gene regulation in the context of nuclear architecture 2 Basal transcriptional machinery 2.1 Core promoter 2.2 TATA box and other core promoter elements 2.3 Genome-wide core promoter identification 2.

4 TFIID and Mediator as paradigms of multi-protein complexes 3 Transcription factors and signal transduction 3.1 Site-specific transcription factors and their domains 3.2 Classification of transcription factors 3.3. Activation of transcription factors 3.4 Programing cellular differentiation by transcription factors 3.5 Inflammatory signaling via NF-B 3.6 Sensing cellular stress via p53 4 A key transcription factor family: Nuclear receptors 4.

1 The nuclear receptor superfamily 4.2 Molecular interactions of nuclear receptors 4.3 Physiological role of nuclear receptors 5 Genome-wide principles of gene regulation 5.1 Next-generation sequencing 5.2 Gene regulation in the context of Big Biology 5.3 Exploring genome-wide transcription factor binding 5.4 Integrating epigenome-wide datasets 6 Chromatin modifiers 6.1 Cytosines and their methylation 6.

2 Histone modifications 6.3 Gene regulation via chromatin modifiers 6.4 Sensing energy metabolism via chromatin modifiers 7 Epigenetics 7.1 Epigenetics and chromatin 7.2 Genome-wide understanding of epigenetics 7.3 CTCF and genetic imprinting 7.4 Epigenetics in health and disease 8 Chromatin remodeling and organization 8.1 Nucleosome positioning at promoters 8.

2 Chromatin remodeling 8.3 Transcriptional dynamics in the presence of chromatin 8.4 Organization of the nucleus 9 Regulatory impact of non-coding RNA 9.1 Non-coding RNAs 9.2 miRNAs and their regulatory potential 9.3 Long ncRNAs 9.4 Enhancer RNAs.