Introduction: The Why's and How's for Studying Spermatogenesis and Spermatogonial Stem Cells.- Optimizing Testicular Fixation-Embedding Procedures for an Improved Morphofunctional Evaluation of Mammalian Spermatogonia.- Single-Molecule Fluorescence In Situ Hybridization for Spatial Detection of mRNAs in Sections of Mammalian Testes.- Bulk and Single-Cell RNA-Seq Analyses for Studies of Spermatogonia.- Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell - Part 1: Epigenomics Assays.- Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell - Part 2: Computational Analysis of Epigenomics Data.- Primary Cultures of Spermatogonia and Testis Cells.- Reconstitution of Human Prospermatogonial Development from Human Induced Pluripotent Stem Cells.
- RNA Interference as a Method of Gene Knockdown in Cultured Spermatogonia.- Isolation of Undifferentiated Spermatogonia from Adult and Developing Mouse Testes.- Spermatogonial Transplantation.- Viral Transduction of Mammalian Spermatogonial Stem Cells.- Pharmacological Treatment of Neonatal and Juvenile Mice to Study Spermatogenesis.- Models and Methods for Evaluating Regeneration of Spermatogenesis After Cytotoxic Treatments.- Defining Gene Function in Spermatogonial Stem Cells through Conditional Knockout Approaches.- Lineage Tracing of Spermatogonial Stem Cells within the Male Germline.
- Perspectives: Approaches for Studying Livestock Spermatogonia.- Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells.